Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil

Abstract

Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.

DOI: 10.1590/0074-02760150053

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Cite this paper

@inproceedings{Santos2015FulllengthIC, title={Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil}, author={Jefferson Jos{\'e} da Silva Santos and Tereza Magalhaes and Jos{\'e} Valter Joaquim Silva and Andr{\'e}a Nazar{\'e} Monteiro Rangel da Silva and Marli Cordeiro and Laura Gil}, booktitle={Memórias do Instituto Oswaldo Cruz}, year={2015} }