Full-length and N-TIMP-3 display equal inhibitory activities toward TNF-alpha convertase.

@article{Lee2001FulllengthAN,
  title={Full-length and N-TIMP-3 display equal inhibitory activities toward TNF-alpha convertase.},
  author={M H Lee and Vera Kn{\"a}uper and J. David Becherer and Gillian Murphy},
  journal={Biochemical and biophysical research communications},
  year={2001},
  volume={280 3},
  pages={945-50}
}
We previously reported that tumor necrosis factor-alpha converting enzyme (TACE) was specifically inhibited by TIMP-3 but not TIMP-1, -2, and -4. Further mutagenesis studies showed that the N-terminal domain of TIMP-3 (N-TIMP-3) retained full inhibitory activity towards TACE. Full-length TIMP-3 and N-TIMP-3 exhibited indistinguishable values for the association rate constant and inhibitory affinity constant for the active catalytic domain of TACE (k(on) approximately 10(5) M(-1) s(-1) and K(app… CONTINUE READING