Full-length RNA-seq from single cells using Smart-seq2

@article{Picelli2014FulllengthRF,
  title={Full-length RNA-seq from single cells using Smart-seq2},
  author={Simone Picelli and Omid R Faridani and {\AA}sa K. Bj{\"o}rklund and G{\"o}sta Winberg and Sven Sagasser and Rickard Sandberg},
  journal={Nature Protocols},
  year={2014},
  volume={9},
  pages={171-181}
}
Emerging methods for the accurate quantification of gene expression in individual cells hold promise for revealing the extent, function and origins of cell-to-cell variability. Different high-throughput methods for single-cell RNA-seq have been introduced that vary in coverage, sensitivity and multiplexing ability. We recently introduced Smart-seq for transcriptome analysis from single cells, and we subsequently optimized the method for improved sensitivity, accuracy and full-length coverage… CONTINUE READING
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