Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung.

@article{Thomas2005FullDO,
  title={Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung.},
  author={Mini Thomas and James J.-Q. Lu and Qing Ge and Chengcheng Zhang and Jianzhu Chen and Alexander M. Klibanov},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2005},
  volume={102 16},
  pages={5679-84}
}
High-molecular-mass polyethylenimines (PEIs) are widely used vectors for nucleic acid delivery. We found that removal of the residual N-acyl moieties from commercial linear 25-kDa PEI enhanced its plasmid DNA delivery efficiency 21 times in vitro, as well as 10,000 times in mice with a concomitant 1,500-fold enhancement in lung specificity. Several additional linear PEIs were synthesized by acid-catalyzed hydrolysis of poly(2-ethyl-2-oxazoline), yielding the pure polycations. PEI87 and PEI217… CONTINUE READING

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