Full-Length gene enrichment by using an optimized RNA isolation protocol in Bixa orellana recalcitrant tissues.

@article{Rodrguezvila2009FullLengthGE,
  title={Full-Length gene enrichment by using an optimized RNA isolation protocol in Bixa orellana recalcitrant tissues.},
  author={Norma Laura Rodr{\'i}guez-{\'A}vila and Jos{\'e} A Narv{\'a}ez-Zapata and Margarita Aguilar-Espinosa and Renata Rivera-Madrid},
  journal={Molecular biotechnology},
  year={2009},
  volume={42 1},
  pages={84-90}
}
A reliable protocol is described for isolation of large full-length cDNA from Bixa orellana mature tissues containing large quantities of pigments, phenols, and polysaccharides. This protocol involves the optimization of a commercial RNA extraction protocol in combination with a long distance reverse transcript PCR protocol. The principal advantages of this protocol are its high RNA yield and quality. The resulting RNA is suitable for RNA expression evaluation and production of large, full… CONTINUE READING

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