Fucose containing oligosaccharides from human milk. I. Separation and identification of new constituents.

  title={Fucose containing oligosaccharides from human milk. I. Separation and identification of new constituents.},
  author={Heinz Egge and Anne Dell and Hubertus Von Nicolai},
  journal={Archives of biochemistry and biophysics},
  volume={224 1},
Neutral oligosaccharides isolated from pooled human milk were subjected to fractionation on high-performance thin-layer chromatography (HPTLC) plates, Iatrobeads, and reverse-phase chromatography after borohydride reduction and peracetylation. By the combined HPLC and HPTLC separation a mixture of pooled human milk oligosaccharides was separated into 101 fractions. These fractions were characterized by field desorption or fast atom bombardment (FAB)-mass spectrometry. Each of the carbohydrate… Expand
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