Fucose containing oligosaccharides from human milk. I. Separation and identification of new constituents.

@article{Egge1983FucoseCO,
  title={Fucose containing oligosaccharides from human milk. I. Separation and identification of new constituents.},
  author={Heinz Egge and Anne Dell and Hubertus Von Nicolai},
  journal={Archives of biochemistry and biophysics},
  year={1983},
  volume={224 1},
  pages={
          235-53
        }
}
Neutral oligosaccharides isolated from pooled human milk were subjected to fractionation on high-performance thin-layer chromatography (HPTLC) plates, Iatrobeads, and reverse-phase chromatography after borohydride reduction and peracetylation. By the combined HPLC and HPTLC separation a mixture of pooled human milk oligosaccharides was separated into 101 fractions. These fractions were characterized by field desorption or fast atom bombardment (FAB)-mass spectrometry. Each of the carbohydrate… Expand
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References

SHOWING 1-10 OF 12 REFERENCES
Heterogeneity of the glycans O-glycosidically linked to the hinge region of secretory immunoglobulins from human milk.
TLDR
Four O-glycosidically linked oligosaccharides linked to serine or threonine residues of the hinge region from myeloma serum immunoglobulin A1 are found to be more complex and heterogenous than the other oligosACcharides isolated from human milk. Expand
Mass spectrometric analysis of permethylated glycosphingolipids II. Comparative studies on different blood-group active and related erythrocyte membrane glycosphingolipids.
TLDR
Three isomeric ceramide tetrasaccharides--P blood- group active globoside, lacto-N-neotetraosyl ceramide as ABH blood-group precursor, both isolated from human erythrocytes and "asialo ganglioside" from human brain as reference standard--were investigated by mass spectrometry after permethylation, and differences in the composition of the ceramide residues can be deduced. Expand
Oligosaccharides of human milk: structural studies of di-and trifucosyl derivatives of lacto-N-octaose and lacto-N-neoctaose.
TLDR
Difucosyl and trifucOSyl derivatives of lacto- N -octaose and lacto -neooctaose were isolated from human milk and their structures were elucidated by sequential enzymic degradation and quantitative methylation analysis. Expand
Fast-atom-bombardment mass-spectrometry for carbohydrate-structure determination
Abstract The potential of fast-atom-bombardment (f.a.b.) mass-spectrometry in the carbohydrate field was assessed with the aid of unmodified, permethylated, and peracetylated oligosaccharides andExpand
Oligosaccharides of human milk: structures of three lacto-N-hexaose derivatives with H-haptenic structure.
TLDR
Three oligosaccharides which have lacto-N-hexaose as their core structures are isolated by borate paper electrophoresis and their structures were elucidated by sequential glycosidase digestion and by methylation analysis. Expand
Oligosaccharides of human milk. Structural studies of two new octasaccharides, difucosyl derivatives of para-lacto-N-hexaose and para-lacto-N-neohexaose.
TLDR
Two new octasaccharides were isolated from human milk of a nonsecretor Lewis a+b- individual and the core portions of these sugars are named para-lacto-N-hexaose and para- lacto -N-neo hexaose, respectively. Expand
Proton-nuclear magnetic resonance study of peracetylated derivatives of ten oligosaccharides isolated from human milk.
TLDR
Proton-nuclear magnetic resonance spectra of peracetylated derivatives of ten structurally related oligosaccharides isolated from human milk were measured for solutions in CDCl3 at 360 MHz and the problems of signal overlap are less severe and the spin systems of all constituent sugar residues can be assigned fully. Expand
Fast atom bombardment of solids as an ion source in mass spectrometry
A new ion source for molecular structure determination of thermolabile and involatile compounds by mass spectrometry is presented together with some preliminary results from peptides, glycosideExpand
Extended performance using a high field magnet mass spectrometer.
The development of a high field magnet for high mass electron impact, chemical ionization, field desorption and fast atom bombardment mass spectrometric studies is described. Its utility isExpand
Fast atom bombardment of solids (F.A.B.): a new ion source for mass spectrometry
A new method for obtaining high-quality mass spectra of molecules which previously were difficult or impossible to study by ionisation methods is described
...
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