Fucogalactan from the giant mushroom Macrocybe titans inhibits melanoma cells migration.

  title={Fucogalactan from the giant mushroom Macrocybe titans inhibits melanoma cells migration.},
  author={Shayane da Silva Milhorini and Fhernanda Ribeiro Smiderle and Stellee Marcela Petris Biscaia and F{\'a}bio R. Rosado and Edvaldo da Silva Trindade and Marcello Iacomini},
  journal={Carbohydrate polymers},
An aqueous extract containing polysaccharides was obtained from the giant mushroom Macrocybe titans, and it was purified by amylase treatment, freeze-thawing process and dialysis. The purified fraction (ESP) was analyzed by HPSEC and GC-MS which showed a homogenous polysaccharide with Mw 14.2 × 103 g/mol composed by galactose and fucose. NMR and methylation analysis of ESP confirmed the presence of a fucogalactan with a (1 → 6)-linked α-d-Galp main chain partially substituted at O-2 by non… Expand
10 Citations
Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus.
A fucomannogalactan obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects and was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells. Expand
In Vitro Antitumor Potential of Sulfated Polysaccharides from Seaweed Caulerpa cupressoides var. flabellata
Results showed that SP inhibited important melanoma development events without cytotoxicity effects, suggesting that C. cupressoides may be an important source of SP with antitumor properties. Expand
Structural characteristics of a hypoglycemic polysaccharide from Fructus Corni.
The results showed that PFC-3 concentration-dependently enhanced glucose uptake and significantly improved glucose consumption in insulin-resistant HepG2 cells, and significantly reduced fasting blood glucose level, glycosylated hemoglobin level, amylase activity, ameliorate lipid metabolism, and hepatic lesions in streptozotocin-induced diabetic rats. Expand
Macrocybin, a mushroom natural triglyceride, reduces tumor growth in vitro and in vivo through caveolin-mediated interference with the actin cytoskeleton
It is shown that Macrocybin constitutes a new biologically active compound that may be taken into consideration for cancer treatment. Expand
Macrocybin, a Natural Mushroom Triglyceride, Reduces Tumor Growth In Vitro and In Vivo through Caveolin-Mediated Interference with the Actin Cytoskeleton
It is shown that Macrocybin constitutes a new biologically active compound that may be taken into consideration for cancer treatment. Expand
Biologically active polysaccharide from edible mushrooms: A review.
The present review offers summarized and significant information about the structural and biological properties of mushroom polysaccharides, and their potential for development of therapeutic materials. Expand
Recent Progress in Research on the Pharmacological Potential of Mushrooms and Prospects for Their Clinical Application
This review discusses recent advances in research on the pharmacological potential of mushrooms and perspectives for their clinical application. Expand
Role of bioactive compounds and other metabolites from mushrooms against skin disorders- a systematic review assessing their cosmeceutical and nutricosmetic outcomes.
It is highlighted that mushrooms contain diverse biomolecules that can be sustainably used in the development of nutricosmetic and cosmeceutical formulations. Expand
An α-D-galactan and a β-D-glucan from the mushroom Amanita muscaria: Structural characterization and antitumor activity against melanoma.
  • Matheus Zavadinack, Daniel de Lima Bellan, +6 authors Marcello Iacomini
  • Medicine, Chemistry
  • Carbohydrate polymers
  • 2021
Polysaccharides α-D-galactan (GAL-Am) and β-D-glucan (GLC-Am) were obtained from Amanita muscaria fruiting bodies. They were purified using different methodologies, such as Fehling precipitation (forExpand


Studies on antioxidative and immunostimulating fucogalactan of the edible mushroom Macrolepiota dolichaula.
The PS-II exhibited the antioxidant properties in different in vitro test systems, and also showed in vitro macrophage activation in RAW 264.7 cell line as well as splenocyte and thymocyte activation in mouse cell culture medium. Expand
Agaricus bisporus fucogalactan: structural characterization and pharmacological approaches.
The present results demonstrate that EFP-Ab could constitute an attractive molecule of interest for the development of new drugs and has significant anti-sepsis, antinociceptive and anti-inflammatory actions, which seems to be related to the decreased iNOS and COX-2 expression. Expand
Antiproliferation of melanoma cells by polysaccharide isolated from Zizyphus jujuba.
The antiproliferation effect of DPP on melanoma cells followed a dose- and time-dependent course and suggested that DPP may be used as a potential anti-skin cancer agent for further in vivo and clinical trial experiments. Expand
Fucogalactan isolated from Sarcodon aspratus elicits release of tumor necrosis factor-alpha and nitric oxide from murine macrophages.
Fucogalactan does elicit the release of cytokines such as TNF-alpha and NO, and shows dose dependence at concentrations from 5 to 500 microg/ml in NO production. Expand
Structural characterization of a novel neutral polysaccharide from Lentinus giganteus and its antitumor activity through inducing apoptosis.
The findings suggest that LGPS-1 induced apoptosis in HepG2 cells through intrinsic mitochondrial apoptosis and PI3K/Akt signaling pathways. Expand
Isolation, purification, structural analysis and immunostimulatory activity of water-soluble polysaccharides from Grifola Frondosa fruiting body.
MTT assay showed that GFP could significantly improve the proliferation activity of RAW264.7 cells in a certain range of concentrations and time and Scanningelectro microscopy results indicated that G FP could induce RAW264,7 cells activation. Expand
Isolation and chemical characterization of a glucogalactomannan of the medicinal mushroom Cordyceps militaris.
Cordyceps militaris dried fruiting bodies was extracted with 5% KOH solution, purified by freeze-thawing treatment, and dialysis, giving rise to a homogeneous polysaccharide, which was identified by NMR and methylation analysis. Expand
Structural characterization and in vitro antitumor activity of a novel polysaccharide isolated from the fruiting bodies of Pleurotus ostreatus.
Cytotoxicity assay showed POPS-1 presented significantly higher antitumor activity against Hela tumor cell in vitro, in a dose-dependent manner, and exhibited significantly lower cytotoxicity to human embryo kidney 293T cells thanHela tumor cells compared with 5-Fu. Expand
Structure of Agaricus spp. fucogalactans and their anti-inflammatory and antinociceptive properties.
The small differences in chemical structure in these polysaccharides thus modified their biological activities and were related to the anti-inflammatory effect. Expand
Purification and partial characterization of a novel anti-tumor glycoprotein from cultured mycelia of Grifola frondosa.
  • F. Cui, X. Zan, +5 authors Ying Dong
  • Biology, Medicine
  • International journal of biological macromolecules
  • 2013
Protein sequencing and 3D model of GFG-3a were finally predicted by using MALDI-TOF-MS, NCBI blast search and online SWISS-MODLE Workspace service and Circular dichroism revealed that GFG -3a was a predominantly β-sheet glycoprotein with a relatively small α-helical content. Expand