FtsZ dimerization in vivo.

  title={FtsZ dimerization in vivo.},
  author={Gustavo Di Lallo and Daniela Anderluzzi and Patrizia Ghelardini and Luciano Paolozzi},
  journal={Molecular microbiology},
  volume={32 2},
A hybrid assay, based on the properties of the lambda repressor, was developed to detect FtsZ dimerization in Escherichia coli in vivo. A gene fusion comprising the N-terminal end of the lambda cI repressor gene and the complete E. coli ftsZ gene was constructed. The fused protein resulted in a functional lambda repressor and was able to complement the thermosensitive mutant ftsZ84. Using the same strategy, a series of 10 novel mutants of FtsZ that are unable to dimerize was selected, and a… CONTINUE READING