From rabbit antibody repertoires to rabbit monoclonal antibodies

  title={From rabbit antibody repertoires to rabbit monoclonal antibodies},
  author={Justus Weber and Haiyong Peng and Christoph Rader},
  journal={Experimental \& Molecular Medicine},
In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe the generation of rabbit monoclonal antibodies by hybridoma technology, phage display and alternative methods, along… 

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Generation and selection of rabbit antibody libraries by phage display.

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Rabbit mAbs are defined reagents with infinite supply, prompting considerable interest in recent years andPhage-display technology based on chimeric rabbit/human Fab libraries allows the selection of rabbit mAbs that cross-react with mouse and human antigens while maintaining high affinity and specificity.

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The method for generating rabbit monoclonal antibodies using the rabbit hybridoma fusion partner 240E-1 is described, which begins with the immunization of rabbits and ends with the cloning the antigen-specific hybridomas.

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The selection of human mAbs from naïve, immune, transgenic, and synthetic human antibody repertoires using methods based on hybridoma technology, clonal expansion of peripheral B cells, single-cell PCR, phage display, yeast display, and mammalian cell display are discussed.

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