From rabbit antibody repertoires to rabbit monoclonal antibodies

@article{Weber2017FromRA,
  title={From rabbit antibody repertoires to rabbit monoclonal antibodies},
  author={Justus Weber and Haiyong Peng and Christoph Rader},
  journal={Experimental \& Molecular Medicine},
  year={2017},
  volume={49}
}
In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe the generation of rabbit monoclonal antibodies by hybridoma technology, phage display and alternative methods, along… 

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References

SHOWING 1-10 OF 193 REFERENCES

Generation and selection of rabbit antibody libraries by phage display.

  • C. Rader
  • Biology
    Methods in molecular biology
  • 2009
Rabbit mAbs are defined reagents with infinite supply, prompting considerable interest in recent years andPhage-display technology based on chimeric rabbit/human Fab libraries allows the selection of rabbit mAbs that cross-react with mouse and human antigens while maintaining high affinity and specificity.

The Rabbit Antibody Repertoire as a Novel Source for the Generation of Therapeutic Human Antibodies*

This work uses phage display technology to select and humanize antibodies from rabbits that were immunized with human A33 antigen which is a target antigen for the immunotherapy of colon cancer.

Generation of rabbit monoclonal antibodies.

The method for generating rabbit monoclonal antibodies using the rabbit hybridoma fusion partner 240E-1 is described, which begins with the immunization of rabbits and ends with the cloning the antigen-specific hybridomas.

Mining human antibody repertoires

The selection of human mAbs from naïve, immune, transgenic, and synthetic human antibody repertoires using methods based on hybridoma technology, clonal expansion of peripheral B cells, single-cell PCR, phage display, yeast display, and mammalian cell display are discussed.

Production and characterization of a rabbit monoclonal antibody against human CDC25C phosphatase.

Although the production of the rabbit MAb requires more effort and patience than the mouse MAb technology, it offers a true alternative in case of antigens that are not immunogenic in mice.

Phage display of combinatorial antibody libraries.

Generation of Rabbit Monoclonal Antibody Fragments from a Combinatorial Phage Display Library and Their Production in the Yeast Pichia pastoris

We have applied the combinatorial immunoglobulin library and phage display technologies to generate monoclonal rabbit single-chain Fv (scFv) antibody fragments specific for recombinant human leukemia
...