From hybridization image to numerical values: a practical, high throughput quantification system for high density filter hybridizations.

Abstract

Hybridization to sets of bacterial colonies or PCR products arrayed on high density filters is used in a number of experimental schemes. In many cases it is desirable to collect quantitative information ('hybridization signatures') rather than indications on 'positive' and 'negative' colonies. We present a practical system, based on an imaging plate… (More)

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