From autophagy to senescence and apoptosis in Angiotensin II-treated vascular endothelial cells.

The aim of this study was to explore if cell autophagy is activated by AngII before aging using human umbilical vascular endothelial cells (HUVECs). The ultrastructural analysis of HUVECs was performed to observe autophagosomes. The LC3-II/LC3-I ratio was determined by western blot assay. The β-gal staining was used to identify cell senescence. The flow… CONTINUE READING