From an electrophoretic mobility shift assay to isolated transcription factors: a fast genomic-proteomic approach

@article{MachAigner2010FromAE,
  title={From an electrophoretic mobility shift assay to isolated transcription factors: a fast genomic-proteomic approach},
  author={A. Mach-Aigner and Karin Grosstessner-Hain and M. Poças-Fonseca and K. Mechtler and R. Mach},
  journal={BMC Genomics},
  year={2010},
  volume={11},
  pages={644 - 644}
}
BackgroundHypocrea jecorina (anamorph Trichoderma reesei) is a filamentous ascomycete of industrial importance due to its hydrolases (e.g., xylanases and cellulases). The regulation of gene expression can influence the composition of the hydrolase cocktail, and thus, transcription factors are a major target of current research. Here, we design an approach for identifying a repressor of a xylanase-encoding gene.ResultsWe used streptavidin affinity chromatography to isolate the Xylanase promoter… Expand
Xpp1 regulates the expression of xylanases, but not of cellulases in Trichoderma reesei
The impact of chromatin remodelling on cellulase expression in Trichoderma reesei
Engineering of the Trichoderma reesei xylanase3 promoter for efficient enzyme expression
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