From Sugar Beet Molasses to Lyphan

  title={From Sugar Beet Molasses to Lyphan},
  author={Robert Faurie and Gerhard Dr. Fries},
  journal={Advances in Experimental Medicine and Biology},
  • R. Faurie, G. Fries
  • Published 1999
  • Chemistry
  • Advances in Experimental Medicine and Biology
L-tryptophan is produced at the AMINO GmbH (Frellstedt, FRG) via biocatalytical condensation of the amino acid L-serine with indole. As a biocatalyst, tryptophan synthetase is used which is produced in high activities by a natural mutant Escherichia coli strain. The enzyme mechanism and specificity and the individual process-parameters for the biotrans-formation procedure are explained as well as the purification process of educts and products. This includes a detailed description of the… Expand
Modelling of an Industrial Biotransformation Process for Tryptophan Production
Abstract For an industrial biotransformation process a simple mathematical model has been developed. During the process indole and serine is converted to tryptophan using the enzyme tryptophanExpand
Aromatic Amino Acids
Biosynthesis of the three aromatic amino acids and its regulation in Escherichia coli and corynebacteria are reviewed and the common aromatic biosynthetic pathway (shikimate pathway) starts with the condensation of phosphoenolpyruvate and erythrose 4-phosphate. Expand
Taproot promoters cause tissue specific gene expression within the storage root of sugar beet
Reporter gene expression analysis in transgenic sugar beet plants revealed that all three promoters are active in the storage root, and the properties of the Mll promoter may be advantageous for the modification of sucrose metabolism in storage roots. Expand
Rapid detection and quantification of Cercospora beticola in soil using PCR and ELISA assays
Two diagnostic methods, polymerase chain reaction (PCR) and Enzyme linked immunoabsorbent assay (ELISA) technique were successfully applied for qualitative and quantitative detection of C. beticola in soil to improve integrated pest management systems of sugar beet. Expand


Production L‐tryptophan by Escherichia coli cells
Whole cells of Escherichia coli B 10 having high tryptophan synthetase activity were used directly as an enzyme source to produce L‐tryptophan from indole and L‐ or D,L‐serine, and among the nonionic detergents, Triton X‐100 was very efficient. Expand