From Proteins to Proteomes: Large Scale Protein Identification by Two-Dimensional Electrophoresis and Arnino Acid Analysis

@article{Wilkins1996FromPT,
  title={From Proteins to Proteomes: Large Scale Protein Identification by Two-Dimensional Electrophoresis and Arnino Acid Analysis},
  author={Marc R. Wilkins and Christian Pasquali and Ron D. Appel and Keli Ou and Olivier Golaz and Jean-Charles Sanchez and Jun X. Yan and Andrew Arthur Gooley and Graham J. Hughes and Ian Humphery-Smith and Keith Leslie Williams and Denis F. Hochstrasser},
  journal={Bio/Technology},
  year={1996},
  volume={14},
  pages={61-65}
}
Separation and identification of proteins by two-dimensional (2-D) electrophoresis can be used for protein-based gene expression analysis. In this report single protein spots, from polyvinylidene difluoride blots of micropreparative E. coli 2-D gels, were rapidly and economically identified by matching their amino acid composition, estimated pI and molecular weight against all E. coli entries in the SWISS-PROT database. Thirty proteins from an E. coli 2-D map were analyzed and identities… 
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