From Proteins to Proteomes: Large Scale Protein Identification by Two-Dimensional Electrophoresis and Arnino Acid Analysis
@article{Wilkins1996FromPT, title={From Proteins to Proteomes: Large Scale Protein Identification by Two-Dimensional Electrophoresis and Arnino Acid Analysis}, author={M. Wilkins and C. Pasquali and R. Appel and K. Ou and O. Golaz and J. Sanchez and J. Yan and A. Gooley and G. Hughes and I. Humphery-Smith and K. Williams and D. Hochstrasser}, journal={Bio/Technology}, year={1996}, volume={14}, pages={61-65} }
Separation and identification of proteins by two-dimensional (2-D) electrophoresis can be used for protein-based gene expression analysis. In this report single protein spots, from polyvinylidene difluoride blots of micropreparative E. coli 2-D gels, were rapidly and economically identified by matching their amino acid composition, estimated pI and molecular weight against all E. coli entries in the SWISS-PROT database. Thirty proteins from an E. coli 2-D map were analyzed and identities… CONTINUE READING
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