From Proteins to Proteomes: Large Scale Protein Identification by Two-Dimensional Electrophoresis and Arnino Acid Analysis

@article{Wilkins1996FromPT,
  title={From Proteins to Proteomes: Large Scale Protein Identification by Two-Dimensional Electrophoresis and Arnino Acid Analysis},
  author={M. Wilkins and C. Pasquali and R. Appel and K. Ou and O. Golaz and J. Sanchez and J. Yan and A. Gooley and G. Hughes and I. Humphery-Smith and K. Williams and D. Hochstrasser},
  journal={Bio/Technology},
  year={1996},
  volume={14},
  pages={61-65}
}
  • M. Wilkins, C. Pasquali, +9 authors D. Hochstrasser
  • Published 1996
  • Biology, Medicine
  • Bio/Technology
  • Separation and identification of proteins by two-dimensional (2-D) electrophoresis can be used for protein-based gene expression analysis. In this report single protein spots, from polyvinylidene difluoride blots of micropreparative E. coli 2-D gels, were rapidly and economically identified by matching their amino acid composition, estimated pI and molecular weight against all E. coli entries in the SWISS-PROT database. Thirty proteins from an E. coli 2-D map were analyzed and identities… CONTINUE READING
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