• DOI:10.1387/ijdb.130205av
• Corpus ID: 12384107

From Agrobacterium to viral vectors: genome modification of plant cells by rare cutting restriction enzymes.

@article{Marton2013FromAT,
  title={From Agrobacterium to viral vectors: genome modification of plant cells by rare cutting restriction enzymes.},
  author={Ira Marton and Arik Honig and Ayelet Omid and N. Tilani S. De Costa and Elena Marhevka and Barry Cohen and Amir Zuker and Alexander Vainstein},
  journal={The International journal of developmental biology},
  year={2013},
  volume={57 6-8},
  pages={
          639-50
        }
}

• I. Marton, A. Honig, +5 authors A. Vainstein
• Published 9 October 2013
• Biology, Engineering
• The International journal of developmental biology

Researchers and biotechnologists require methods to accurately modify the genome of higher eukaryotic cells. Such modifications include, but are not limited to, site-specific mutagenesis, site-specific insertion of foreign DNA, and replacement and deletion of native sequences. Accurate genome modifications in plant species have been rather limited, with only a handful of plant species and genes being modified through the use of early genome-editing techniques. The development of rare-cutting restriction enzymes as a tool for the induction of site-specific genomic double-strand breaks and their introduction as a reliable tool for genome modification in animals, animal cells and human cell lines have paved the way for the adaptation of rare-cutting restriction enzymes to genome editing in plant cells. Indeed, the number of plant species and genes which have been successfully edited using zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and engineered homing endonucleases is on the rise. In our review, we discuss the basics of rare-cutting restriction enzyme-mediated genome-editing technology with an emphasis on its application in plant species.