From ‘protein’ to the beginnings of clinical proteomics

@article{Perrett2007FromT,
  title={From ‘protein’ to the beginnings of clinical proteomics},
  author={David Perrett},
  journal={PROTEOMICS – Clinical Applications},
  year={2007},
  volume={1}
}
  • D. Perrett
  • Published 1 August 2007
  • Biology, Chemistry
  • PROTEOMICS – Clinical Applications
It has taken over 200 years to move from the view that there was a single unique ‘protein’ to our present understanding that there may be over a million proteins in the human body. This review details the technological developments that led to this increase in knowledge especially in the area of clinical biochemistry. Simple techniques such as total protein assays allowed the quantitation of the amounts of protein in blood plasma and serum. These techniques were then linked to simple… 
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References

SHOWING 1-10 OF 112 REFERENCES

The Human Plasma Proteome

TLDR
This work speculates on the reasons behind this large discrepancy between the expectations arising from proteomics and the realities of clinical diagnostics and suggests approaches by which protein-disease associations may be more effectively translated into diagnostic tools in the future.

Chromatographic and Electrophoretic Techniques

TLDR
The reviewer was left with the impression that more attention is paid to these tests in the author's departments than is usually done in Great Britain.

Grouped Variations in the Occurrence of New Protein Components in Normal Human Serum

TLDR
A method of zone electrophoresis using starch gel as the supporting medium and the convenience of protein detection by staining methods is developed, with a resolving power in some cases superior to that obtained in free-solution boundary-electrophoresIS.

Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues

  • J. Klose
  • Medicine, Biology
    Humangenetik
  • 2004
TLDR
The fact that a high number of protein spots can be evaluated by a single and comparatively simple experiment suggests that this method may be useful as an assay system for induced point mutations.

Determination of serum proteins by means of the biuret reaction.

Electrophoresis: the march of pennies, the march of dimes.

Zone electrophoresis in starch gels and its application to studies of serum proteins.

  • O. Smithies
  • Chemistry, Medicine
    Advances in protein chemistry
  • 1959

A study of the partial acid hydrolysis of some proteins, with special reference to the mode of linkage of the basic amino-acids.

TLDR
It seems clear that the distinctive properties of proteins reside in the arrangement rather than the nature of the amino-acid residues, and the only direct evidence in favour of a hypothesis concerning the order of amino- acid residues in a peptide chain can come from the isolation and identification of fragments of this chain containing at least two amino- Acid residues.

Progress with gene‐product mapping of the Mollicutes: Mycoplasma genitalium

TLDR
A protein map of the smallest known self‐replicating organism, Mycoplasma genitalium, revealed a high proportion of acidic proteins, which allowed proteins to be identified prior to detection of their respective genes via the M. genitalium sequencing initiative.

Joachim kohn (1912-1987) and the origin of cellulose acetate electrophoresis.

TLDR
This short review highlights Joachim Kohn's discovery of CA electrophoresis and his contributions to the development of this procedure.
...