Fretting about FRET: correlation between kappa and R.

  title={Fretting about FRET: correlation between kappa and R.},
  author={Darren B VanBeek and Matthew C. Zwier and Justin M. Shorb and Brent P. Krueger},
  journal={Biophysical journal},
  volume={92 12},
Molecular dynamics simulations were used to examine the structural dynamics of two fluorescent probes attached to a typical protein, hen egg-white lysozyme (HEWL). The donor probe (D) was attached via a succinimide group, consistent with the commonly-used maleimide conjugation chemistry, and the acceptor probe (A) was bound into the protein as occurs naturally for HEWL and the dye Eosin Y. The is found to deviate significantly from the theoretical value and high correlation between the… CONTINUE READING