Fresh islets are more effective for islet transplantation than cultured islets.

@article{Noguchi2012FreshIA,
  title={Fresh islets are more effective for islet transplantation than cultured islets.},
  author={Hirofumi Noguchi and Bashoo Naziruddin and Andrew M Jackson and Masayuki Shimoda and Tetsuya Ikemoto and Yasutaka Fujita and Daisuke Chujo and Morihito Takita and Han Peng and Koji Sugimoto and Takeshi Itoh and Naoya Kobayashi and Nicholas Onaca and Marlon F. Levy and Shinichi Matsumoto},
  journal={Cell transplantation},
  year={2012},
  volume={21 2-3},
  pages={517-23}
}
For clinical islet transplantation, isolated islets deteriorate rapidly in culture, although culturing islets prior to transplantation provides flexibility for evaluation of isolated islets and pretreatment of patients. In the present study, we compared human fresh islets to cultured islets with in vitro and in vivo assays. After culture for 24, 48, and 72 h, islet yield significantly decreased from 2,000 to 1,738 ± 26 (13% loss), 1,525 ± 30 (24% loss), or 1,298 ± 18 IEQ (35% loss… CONTINUE READING