Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia.

Abstract

Micro-RNAs (miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors ( approximately 70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target mRNAs via base-pairing interactions. Here we show that miR15 and miR16 are located at chromosome 13q14, a region deleted in more than half of B cell chronic lymphocytic leukemias (B-CLL). Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority ( approximately 68%) of CLL cases.

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@article{Calin2002FrequentDA, title={Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia.}, author={George A. Calin and Calin Dan Dumitru and Masayoshi Shimizu and Roberta Bichi and Simona Zupo and Evan K Noch and Hansjuerg Aldler and Sashi Rattan and Michael J Keating and Kanti R Rai and Laura Rassenti and Thomas J. Kipps and Massimo Negrini and Florencia Bullrich and Carlo M. Croce}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={2002}, volume={99 24}, pages={15524-9} }