Frequent coinfection with hepatitis B virus strains of distinct genotypes detected by hybridization with type-specific probes immobilized on a solid-phase support.

@article{Kato2003FrequentCW,
  title={Frequent coinfection with hepatitis B virus strains of distinct genotypes detected by hybridization with type-specific probes immobilized on a solid-phase support.},
  author={Hideaki Kato and Etsuro Orito and Fuminaka Sugauchi and Ryuzo Ueda and Takuya Koshizaka and Shoko Yanaka and Robert Gish and Fuat Kurbanov and Ruslan Ruzibakiev and Anna Kramvis and Michael Charles Kew and Nooruddin Ahmad and Mobin Khan and Sadakazu Usuda and Yuzo Miyakawa and Masashi Mizokami},
  journal={Journal of virological methods},
  year={2003},
  volume={110 1},
  pages={29-35}
}
A genotype-specific probes assay (GSPA) was developed for distinguishing the seven genotypes (A-G) of hepatitis B virus (HBV). Nucleotide (nt) sequences corresponding to preS1 region were amplified by PCR with a primer labeled with biotin, and delivered to eight wells on which complementary sequences specific to one or other genotype had been immobilized. Thereafter, hybridization of HBV DNA sequences amplified from the test serum was detected by colorimetry. When 256 sera from HBV carriers in… CONTINUE READING

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