Frequent Detection and Genetic Diversity of Human Bocavirus in Urban Sewage Samples

@article{Iaconelli2016FrequentDA,
  title={Frequent Detection and Genetic Diversity of Human Bocavirus in Urban Sewage Samples},
  author={Marcello Iaconelli and Maurizio Divizia and Simonetta della Libera and Paola di Bonito and Giuseppina La Rosa},
  journal={Food and Environmental Virology},
  year={2016},
  volume={8},
  pages={289-295}
}
The prevalence and genetic diversity of human bocaviruses (HBoVs) in sewage water samples are largely unknown. In this study, 134 raw sewage samples from 25 wastewater treatment plants (WTPs) in Italy were analyzed by nested PCR and sequencing using species-specific primer pairs and broad-range primer pairs targeting the capsid proteins VP1/VP2. A large number of samples (106, 79.1 %) were positive for HBoV. Out of these, 49 were classified as HBoV species 2, and 27 as species 3. For the… CONTINUE READING

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