Frequency of bacille Calmette-Guerin (BCG) and Mycobacterium tuberculosis in tissue biopsy specimens of children vaccinated with BCG.

  title={Frequency of bacille Calmette-Guerin (BCG) and Mycobacterium tuberculosis in tissue biopsy specimens of children vaccinated with BCG.},
  author={Maryam Monajemzadeh and Reza Shahsiah and Abdolmajid Zarei and Alireza Alai Alamooti and Fatemeh Elham Mahjoub and Setareh Mamishi and Ghamartaj Khotai and Reza Pazira and Neda Eram},
  journal={American journal of clinical pathology},
  volume={133 1},
Vaccination of all newborns with bacille Calmette-Guérin (BCG) vaccine is a standard practice in developing countries. Disseminated mycobacterial infection in an immunocompromised child can be caused by BCG and other mycobacteria. A total of 21 patients with a histopathologic diagnosis of mycobacterial infection were studied in a period of 4 years. DNA was extracted from formalin-fixed, paraffin-embedded tissues. Real-time polymerase chain reaction was performed to determine the mycobacterial… 
Development of a multiplex real-time PCR assay for BCG and validation in a clinical laboratory
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Development of a Multiplex Real-Time PCR Assay for Mycobacterium bovis BCG and Validation in a Clinical Laboratory
This study describes the development of a two-step multiplex real-time PCR assay which uses single nucleotide polymorphisms to detect BCG and identify early or late BCG strains, and can reliably identify BCG from bacterial culture or directly from infected tissue.
Development of a Multiplex Real-Time PCR Assay for <named-content content-type='genus-species'>Mycobacterium bovis</named-content> BCG and Validation in a Clinical Laboratory
A two-step multiplex real-time PCR assay which uses single nucleotide polymorphisms to detect BCG and identify early or late BCG strains and can also differentiate between strains of BCG, which have been suggested to be associated with different rates of adverse events.
Two cases of disseminated BCG disease following vaccination in the same family: case reports and review of the literature in China
Vaccination of infants with BCG is cost-effective and should not be stopped because of the possibility of rare disseminated BCG disease, especially in countries with high tuberculosis burdens such as China, however, infants with a family history of immunodeficiency should be vaccinated after excluding immunODeficiency-related diseases to minimize the risk of disseminatedBCG disease.
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BCG Vaccination as a Prevention Strategy, Threats and Benefits
It has the potential to cause disseminated infection, and an increasing number of immunocompromised patients prone to disease and the suboptimal preventive potency of this vaccine suggest the need for a global attempt to review its benefits and disadvantages.
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Bacille Calmette-Guérin vaccine-induced disease in HIV-infected and HIV-uninfected children.
The presentation treatment and mortality of children who develop BCG disease with emphasis on HIV-infected children are reported and a revised classification ofBCG disease in children is presented and standard diagnostic and management guidelines are proposed.
Disseminated Mycobacterium bovis infection after BCG vaccination.
Dseminated BCG disease is an uncommon but devastating complication of vaccination that should be considered in the appropriate clinical setting and Immune-compromised infants are at greatest risk and they respond poorly to standard therapies.
Disseminated bacille Calmette-Guérin disease after vaccination: case report and review.
Disseminated BCG disease has historically been a disease of infants, but cases now occur in adults and older children coinfected with human immunodeficiency virus, and cases also occur after revaccination of individuals who were anergic following the initial administration of BCG vaccine.
Idiopathic disseminated bacillus Calmette-Guérin infection: a French national retrospective study.
Iiopathic disseminated BCG infection is a rare but severe complication of BCG vaccination that probably results from an as yet unknown genetically determined immunodeficiency condition that affects the killing of intracellular bacteria such as BCG and Salmonella.
PCR identification of Mycobacterium bovis BCG
A multiplex PCR method can be used as a tool for the rapid and specific identification of the attenuated bacillus Calmette-Guérin vaccine strain.
Disseminated bacillus Calmette-Guérin infections in patients with primary immunodeficiencies.
In the later stages of disease or in severe immunodeficiency, there is a lack of granuloma formation and unimpeded proliferation of AFB, reminiscent of nontuberculous mycobacterial infections in AIDS patients.
Pathologic findings for bacille Calmette-Guérin infections in immunocompetent and immunocompromised patients.
  • L. A. al-Bhlal
  • Medicine, Biology
    American journal of clinical pathology
  • 2000
The pathologic findings from biopsy specimens from 9 patients with postvaccination bacille Calmette-Guérin (BCG) infection are presented and patients with normal immunity and immunocompromised patients, who were immunosuppressed, are presented.
The phenotypic heterogeneity of the course of BCG infection reflects distinct pathogenic mechanisms and probably results from a genotypicogeneity of the underlying inherited immune disorder.
Complications of bacille Calmette-Guérin (BCG) vaccination and immunotherapy and their management.
  • J. Grange
  • Medicine
    Communicable disease and public health
  • 1998
BCG should never be given to people who are known to be infected with HIV, but the risk of complications in children born to HIV infected mothers is low, and surgery may be needed for suppurating or discharging lesions to hasten recovery and give a good cosmetic result.
Polymerase chain reaction to detect Mycobacterium tuberculosis in histologic specimens.
Data indicate that PCR amplification is useful for detecting M. tuberculosis DNA in formalin-fixed tissue specimens, and that it can be used to increase diagnostic accuracy in patients who have perplexing diagnostic problems associated with a granulomatous tissue response.