Frequency decoding of fast calcium oscillations by calpain.

  title={Frequency decoding of fast calcium oscillations by calpain.},
  author={Peter Tompa and R T{\"o}th-Bocon{\'a}di and P{\'e}ter Friedrich},
  journal={Cell calcium},
  volume={29 3},
We report the development of a novel procedure for generating fast, high-frequency Ca2+ oscillations in vitro and the frequency-dependent activation of m-calpain, the Ca2+-activated intracellular cysteine protease. The procedure is based upon liberating Ca2+ from a cage, DM-Nitrophen, by repetitive UV laser pulses and its concomitant binding by a 'slow' chelator, 1,4,7,10-tetraazacyclododecane-N,N',N",N"'-tetraacetate (DOTA). It is shown that a full control over the pattern of oscillations can… CONTINUE READING


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