Frequency, size, and localization of bacterial aggregates on bean leaf surfaces.

  title={Frequency, size, and localization of bacterial aggregates on bean leaf surfaces.},
  author={J M Monier and Steven E. Lindow},
  journal={Applied and environmental microbiology},
  volume={70 1},
Using epifluorescence microscopy and image analysis, we have quantitatively described the frequency, size, and spatial distribution of bacterial aggregates on leaf surfaces of greenhouse-grown bean plants inoculated with the plant-pathogenic bacterium Pseudomonas syringae pv. syringae strain B728a. Bacterial cells were not randomly distributed on the leaf surface but occurred in a wide range of cluster sizes, ranging from single cells to over 10(4) cells per aggregate. The average cluster size… CONTINUE READING

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