Freezing-induced uptake of trehalose into mammalian cells facilitates cryopreservation.

  title={Freezing-induced uptake of trehalose into mammalian cells facilitates cryopreservation.},
  author={Miao Zhang and Harri{\"e}tte Oldenhof and Harald Sieme and Willem F Wolkers},
  journal={Biochimica et biophysica acta},
  volume={1858 6},
The aim of this study was to investigate if membrane-impermeable molecules are taken up by fibroblasts when exposing the cells to membrane phase transitions and/or freezing-induced osmotic forces. The membrane-impermeable fluorescent dye lucifer yellow (LY) was used to visualize and quantify uptake during endocytosis, and after freezing-thawing. In addition, trehalose uptake after freezing and thawing was studied. Fourier transform infrared spectroscopic studies showed that fibroblasts display… CONTINUE READING
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Use of Fourier transform infrared spectroscopy to determine optimal cooling rates for cryopreservation of cells

  • H. Oldenhof, M. Akhoondi, H. Sieme, W. F. Wolkers
  • Biomedical Spectroscopy and Imaging 2
  • 2013
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