Freezing Tolerance in Plants Requires Lipid Remodeling at the Outer Chloroplast Membrane

  title={Freezing Tolerance in Plants Requires Lipid Remodeling at the Outer Chloroplast Membrane},
  author={Eric R. Moellering and Bagyalakshmi Muthan and Christoph Benning},
  pages={226 - 228}
Freezing Tolerance Explained Freezing temperatures exact a toll on plant cells through various mechanisms, including disruption of water balances as ice crystals form. Cellular and organelle lipid bilayers are also put under stress. Moellering et al. (p. 226, published online 26 August; see the Perspective by Browse) analyzed the function of a protein in the model plant, Arabidopsis thaliana that, when disrupted, leaves plants more susceptible to damage by freezing. The protein, SENSITIVE TO… 

Chloroplast Membrane Remodeling during Freezing Stress Is Accompanied by Cytoplasmic Acidification Activating SENSITIVE TO FREEZING21[OPEN]

It is concluded that pH and Mg2+ are perceived as intracellular cues as part of the sensing mechanism for freezing conditions, providing a specific molecular mechanism to combat freezing.

Differential remodeling of the lipidome during cold acclimation in natural accessions of Arabidopsis thaliana.

An alternative ecological genomics approach that relies on the natural genetic variation within a species, highly correlated with the freezing tolerance of the accessions, allowing the identification of possible marker lipids for plant freezing tolerance.

Membrane Lipid Remodeling in Response to Salinity

This review provides a comprehensive overview of the recent works that have been carried out on lipid remodeling of plant membranes under salt treatment and the role of membrane lipids as signaling molecules in response to salinity is discussed.

Specific Changes in Arabidopsis thaliana Rosette Lipids during Freezing Can Be Associated with Freezing Tolerance

While the roles of a few specific lipids in plant freezing tolerance are understood, the effect of many plant lipids remains to be determined. Acclimation of plants to non-freezing cold before

Lipidomic studies of membrane glycerolipids in plant leaves under heat stress.

EARLY RESPONSE TO DEHYDRATION 7 Remodels Cell Membrane Lipid Composition During Cold Stress in Arabidopsis.

It is shown that ERD7 and its homologs are important for plant stress responses and development and associated with modification of membrane lipid composition.

SENSITIVE TO FREEZING2 Aids in Resilience to Salt and Drought in Freezing-Sensitive Tomato1[OPEN]

Discovery of tomato SFR2 function in drought and salt resilience provides further insights into general membrane lipid remodeling-based stress tolerance mechanisms and together with protection against freezing in freezing-resistant plants such as Arabidopsis, it adds lipid remodels as a possible target for the engineering of abiotic stress-resilient crops.

Long-chain base kinase1 affects freezing tolerance in Arabidopsis thaliana.

Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation

It is suggested that the remodeling of membrane lipids and attenuation of lipid degradation are critical for the successful use of PVS, and these new insights provide a framework for technology development for the preservation of other species at increasing risk of extinction.

Identification of Chloroplast Envelope Proteins with Critical Importance for Cold Acclimation1[OPEN]

Analysis of the frost recovery of ntt loss-of-function and mex1 overexpressor mutants confirmed that the comparative proteome is well suited to identify novel key factors involved in cold acclimation and acquisition of freezing tolerance.



A role for SENSITIVE TO FREEZING2 in protecting chloroplasts against freeze-induced damage in Arabidopsis.

It is shown that homologous genes are present, and expressed, in a wide range of terrestrial plants, including species not able to tolerate freezing, and expression constructs derived from the cDNAs of a number of different plant species are able to complement the freezing sensitivity of the Arabidopsis sfr2 mutant.

PLANT COLD ACCLIMATION: Freezing Tolerance Genes and Regulatory Mechanisms.

  • M. Thomashow
  • Environmental Science, Biology
    Annual review of plant physiology and plant molecular biology
  • 1999
This review of recent advances in determining the nature and function of genes with roles in freezing tolerance and the mechanisms involved in low temperature gene regulation and signal transduction concludes that cold acclimation includes the expression of certain cold-induced genes that function to stabilize membranes against freeze-induced injury.

Mode of action of the COR15a gene on the freezing tolerance of Arabidopsis thaliana.

It is proposed that COR15am, which is located in the chloroplast stroma, defers freeze-induced formation of the hexagonal II phase to lower temperatures (lower hydrations) by altering the intrinsic curvature of the inner membrane of the chloroplasts envelope.

Cold Acclimation of Arabidopsis thaliana (Effect on Plasma Membrane Lipid Composition and Freeze-Induced Lesions)

Maximum freezing tolerance of Arabidopsis thaliana L. Heyn (Columbia) was attained after 1 week of cold acclimation at 2[deg]C. During this time, there were significant changes in both the lipid

Differential Degradation of Extraplastidic and Plastidic Lipids during Freezing and Post-freezing Recovery in Arabidopsis thaliana*

Data suggest a negative role for PLDα1 and a positive role forPLDδ in freezing tolerance, and distinctly different changes in lipid hydrolysis occur in freezing and post-freezing recovery.

The SENSITIVE TO FREEZING2 Gene, Required for Freezing Tolerance in Arabidopsis thaliana, Encodes a β-Glucosidase

After purification from a heterologous expression system, AtSFR2 displayed a specific hydrolytic activity against β-d-glucosides, and its identical freezing-sensitive phenotype confirmed that the SFR2 gene product is essential for freezing tolerance.

A prominent role for the CBF cold response pathway in configuring the low-temperature metabolome of Arabidopsis.

The results indicate that the metabolome of Arabidopsis is extensively reconfigured in response to low temperature, and that the CBF cold response pathway has a prominent role in this process.

Research on plant abiotic stress responses in the post-genome era: past, present and future.

Recent progress in abiotic stress studies, especially in the post-genomic era, is summarized, new perspectives on research directions for the next decade are offered, and the availability of the complete genome sequence has facilitated access to essential information.

Freezing Sensitivity in the sfr4 Mutant of Arabidopsis Is Due to Low Sugar Content and Is Manifested by Loss of Osmotic Responsiveness1

Results indicate that the freezing sensitivity of cold-acclimated sfr4 is due to its continued susceptibility to LOR (associated with lyotropic formation of the hexagonal II phase) and associated with the low sugar content of its cells.

Depletion of the Membrane-Associated Acyl-Coenzyme A-Binding Protein ACBP1 Enhances the Ability of Cold Acclimation in Arabidopsis1[OA]

ACBP1 overexpressors demonstrated increased freezing sensitivity accompanied by a decrease in PC and an increase in phosphatidic acid (PA), while acbp1 mutant plants showed enhanced freezing tolerance associated with PC accumulation and PA reduction, suggesting that the ACBP1-mediated response is PLD associated and is independent of osmolyte accumulation.