Freeze-thaw and high-voltage discharge allow macromolecule uptake into ileal brush-border vesicles.

@article{Donowitz1987FreezethawAH,
  title={Freeze-thaw and high-voltage discharge allow macromolecule uptake into ileal brush-border vesicles.},
  author={Mark Donowitz and Eugene Emmer and J D McCullen and L J Reinlib and Michael Enrique Cohen and Richard P. Rood and James L. Madara and Geoffrey W. G. Sharp and Heini Murer and K Malmstrom},
  journal={The American journal of physiology},
  year={1987},
  volume={252 6 Pt 1},
  pages={G723-35}
}
High-voltage discharge or one cycle of freeze-thawing are shown to transiently permeabilize rabbit ileal brush-border membrane vesicles to macromolecules. Uptake of the radiolabeled macromolecule dextran, mol wt 70,000, used as a marker for vesicle permeability, was determined by a rapid filtration technique, with uptake defined as substrate associated with the vesicle and releasable after incubation of vesicles with 0.1% saponin. Dextran added immediately after electric shock (2,000 V) or at… CONTINUE READING
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