Freeze-substitution without aldehyde or osmium fixatives: ultrastructure and implications for immunocytochemistry.

@article{Monaghan1990FreezesubstitutionWA,
  title={Freeze-substitution without aldehyde or osmium fixatives: ultrastructure and implications for immunocytochemistry.},
  author={Paul F. Monaghan and David P. Robertson},
  journal={Journal of microscopy},
  year={1990},
  volume={158 Pt 3},
  pages={355-63}
}
Cryo-fixation followed by freeze-substitution without aldehyde or osmium fixation has been investigated as a method for preparing biological specimens with a view to minimizing antigenic alteration. Samples of both solid tissues (mouse small intestine and human kidney) and a human tumour cell line grown in vitro were rapidly frozen by impact (slammed) onto… CONTINUE READING