Freeze-fracture of 2-cell mouse embryos. A new method for fracture of very small and scarce biological samples.

Abstract

Membrane morphology and development of intercellular junctions in 2-cell mouse embryos was studied with freeze-fracture electron microscopy. In order to circumvent the dual problem of relative scarcity of samples and their very small size (about 50 micrometers per blastomere), both of which made conventional microtomy-induced freeze-cleavage impractical, a… (More)

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