Freeze-fracture cytochemical study of membrane systems in human epidermis using filipin as a probe for cholesterol.

Filipin (a polyene antibiotic) interacts specifically with cholesterol in membranes, producing characteristic 25 nm-diameter deformation (pitlike lesions) within the membrane plane detectable by freeze-fracture electron microscopy. Utilizing this probe, the distribution of cholesterol molecules in membranes and in lamellar structures between horny cells was… CONTINUE READING