Freeze-drying of a purified human diploid cell rabies vaccine.

  title={Freeze-drying of a purified human diploid cell rabies vaccine.},
  author={Mirko Majer and Annelies Herrmann and J W Hilfenhaus and Ewa Reichert and Rudolph F. Mauler and Walter A. Hennessen},
  journal={Developments in biological standardization},
A rabies vaccine prepared in human diploid cell strains was purified by continuous-flow sucrose density gradient centrifugation. The peak fractions containing 35-40% sucrose were diluted with a stabilizer consisting of degraded gelatine in a Tris-EDTA-buffer, and then inactivated with beta-propiolactone and freeze-dried to a residual moisture of… CONTINUE READING