Free radical-induced site-specific peptide cleavage in the gas phase: low-energy collision-induced dissociation in ESI- and MALDI mass spectrometry.

Abstract

Protein identification is routinely accomplished by peptide sequencing using mass spectrometry (MS) after enzymatic digestion. Site-specific chemical modification may improve peptide ionization efficiency or sequence coverage in mass spectrometry. We report herein that amino group of lysine residue in peptides can be selectively modified by reaction with a… (More)

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