Free fatty acids increase cytosolic free calcium and stimulate insulin secretion from beta-cells through activation of GPR40.

@article{Schnell2007FreeFA,
  title={Free fatty acids increase cytosolic free calcium and stimulate insulin secretion from beta-cells through activation of GPR40.},
  author={Susanne Schnell and Michael Schaefer and Christof Sch{\"o}fl},
  journal={Molecular and cellular endocrinology},
  year={2007},
  volume={263 1-2},
  pages={173-80}
}
Free fatty acids (FFA) cause a rise in cytosolic free Ca2+ ([Ca2+]i) and stimulate insulin release from pancreatic beta-cells. The G-protein coupled receptor GPR40 can be activated by medium- and long-chain FFA. We investigated a potential role for GPR40 in the generation of the FFA-induced Ca2+ signal and insulin secretion. [Ca2+]i was measured in primary mouse beta-cells and in INS-1 cells, and insulin secretion was assessed from INS-1 cells. GPR40 expression was determined by RT-PCR and… CONTINUE READING

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GPR40 expression was determined by RT - PCR and downregulation of GPR40 expression by siRNA transfection was carried out in INS-1 cells .
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