Free-Solvent Model of Osmotic Pressure Revisited: Application to Concentrated IgG Solution under Physiological Conditions

@article{Yousef1998FreeSolventMO,
  title={Free-Solvent Model of Osmotic Pressure Revisited: Application to Concentrated IgG Solution under Physiological Conditions},
  author={Yousef and Datta and Rodgers},
  journal={Journal of colloid and interface science},
  year={1998},
  volume={197 1},
  pages={108-18}
}
The osmotic pressure measurements of bovine immuno-gamma globulin in phosphate-buffered solution at pH 7.4 and 0.13 M total salt concentration were extended to near saturation concentrations for ambient temperature. The osmotic pressure at the highest measured concentration of 424 g/L was 4.18 psi (28.3 kPa). A free-solvent model, considering solute-solvent interaction in the concentration variable, provided an excellent fit to observed osmotic pressure nonideality at even the highest protein… CONTINUE READING