Framework for using quantitative PCR as a nonculture based method to estimate virus infectivity.

@article{Pecson2011FrameworkFU,
  title={Framework for using quantitative PCR as a nonculture based method to estimate virus infectivity.},
  author={Brian M. Pecson and Martin Ackermann and Tamar Kohn},
  journal={Environmental science & technology},
  year={2011},
  volume={45 6},
  pages={2257-63}
}
Measuring the efficiency of virus disinfection with quantitative PCR (qPCR) has been criticized as inadequate due to the production of false-positive signals. Such a claim, however, presupposes an understanding of the theoretical qPCR response. Many studies have assumed that the loss in qPCR signal upon disinfection should equal the loss in infectivity, without accounting for the fact that qPCR typically assays only a fraction of the viral genome. This study aimed to develop a theoretical… CONTINUE READING
22 Citations
22 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 22 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 22 references

Similar Papers

Loading similar papers…