Frameshifting is required for production of the transposase encoded by insertion sequence 1.

@article{Sekine1989FrameshiftingIR,
  title={Frameshifting is required for production of the transposase encoded by insertion sequence 1.},
  author={Yasuji Sekine and Eiichi Ohtsubo},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1989},
  volume={86 12},
  pages={4609-13}
}
Insertion sequence IS1 has two coding frames, insA and insB, which are essential for its transposition. Here, we show that a frameshifting event in the -1 direction from the 3' end region of the insA frame to an open reading frame (B' frame), extending from the 5' end of the insB frame, is involved in production of the InsA-B'-InsB fusion protein that has IS1 transposase activity. The frameshifting event is likely to have occurred at the sequence AAAAAC where the insA frame overlaps the B… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 32 extracted citations

Similar Papers

Loading similar papers…