Fracture damage of embryos and its prevention during vitrification and warming.

@article{Kasai1996FractureDO,
  title={Fracture damage of embryos and its prevention during vitrification and warming.},
  author={Magosaburo Kasai and Shi En Zhu and Prudencio B. Pedro and Koji Nakamura and Toshihiro Sakurai and Keisuke Edashige},
  journal={Cryobiology},
  year={1996},
  volume={33 4},
  pages={459-64}
}
The frequency of fracture damage in mouse blastocysts was examined by repeated cycles of vitrification and warming. Mouse blastocysts suspended in a solution of ethylene glycol, Ficoll, and sucrose in a straw were plunged into liquid nitrogen either directly (rapidly) or after holding them in liquid nitrogen vapor for 3 min or more (moderately). Vitrified samples were warmed by plunging them into 25 degrees C water either immediately (rapidly) or after holding in air for 5-30 s (moderately… CONTINUE READING

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