Fractionation of the proteins of plant microbodies.

  title={Fractionation of the proteins of plant microbodies.},
  author={Richard H. Brown and J. Michael Lord and Michael J. Merrett},
  journal={The Biochemical journal},
  volume={144 3},
1. Glyoxysomes and peroxisomes have been isolated from dark- and light-grown seedlings of pumpkin (Cucurbita pepo) by sucrose-density-gradient centrifugation. 2. Pumpkin microbodies and castor-bean (Ricinus communis) glyoxysomes may be fractionated, by a combination of osmotic shock and treatment with KCl, into three distinct groups of proteins: readily soluble (matrix enzymes), solubilized in the presence of KCl (membrane-bound enzymes) and relatively insoluble (membrane ;ghost' proteins). 3… CONTINUE READING