Fractionation of complex protein mixtures by liquid-phase isoelectric focusing.

  title={Fractionation of complex protein mixtures by liquid-phase isoelectric focusing.},
  author={Julie Hey and A. E. Posch and Andrew Cohen and Ning Liu and Adrianna Harbers},
  journal={Methods in molecular biology},
Protein fractionation is essential to uncovering low-abundance proteins in complex protein mixtures. Many common methods and techniques are used to fractionate proteins, including chromatography (size exclusion, affinity, ion exchange, etc.), electrophoresis, and solution chemistry. Regardless of the method employed, the ultimate goal of protein fractionation is to enable more protein analysis by today's current proteomics technologies, such as one- (1-DGE) or two-dimensional gel… CONTINUE READING


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First-dimension separation with the MicroRotofor cell prior to SDS-PAGE and LC-MS/MS analysis

L. V. Bindschedler, M. Palmblad, R. Cramer
Bulletin 5451, • 2006
View 2 Excerpts

Development of a two-dimensional protein-peptide separation protocol for comprehensive proteome measurements.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences • 2003
View 1 Excerpt

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