Fractionation of chromatin based on strength of binding with the matrix

Abstract

By washing and subsequent centrifugation of rat liver nuclei in medium of low ionic strength the chromatin can be separated into two fractions: Chromatin eluting into the supernatant and chromatin firmly bound with the matrix. The chromatin fraction eluting into the supernatant contains about 60–70% of the original DNA and about 15% of the original protein… (More)
DOI: 10.1007/BF00831000

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Cite this paper

@article{Mironov2004FractionationOC, title={Fractionation of chromatin based on strength of binding with the matrix}, author={Nikolai M. Mironov and Victor V. Lobanenkov and V. S. Shapot}, journal={Bulletin of Experimental Biology and Medicine}, year={2004}, volume={89}, pages={136-138} }