Fractionation Protocol for the Isolation of Polypeptides from Plant Biomass

@article{Claeson1998FractionationPF,
  title={Fractionation Protocol for the Isolation of Polypeptides from Plant Biomass},
  author={Claeson and G{\"o}ransson and Johansson and Luijendijk and Bohlin},
  journal={Journal of natural products},
  year={1998},
  volume={61 1},
  pages={77-81}
}
A fractionation protocol for the isolation of a highly purified polypeptide fraction from plant biomass is described. The procedure dereplicates ubiquitous substance classes known to interfere with bioassays often used in natural product-based drug discovery programs. The protocol involves pre-extraction with dichloromethane, extraction with ethanol (50%), removal of tannins with polyamide, removal of low-molecular-weight components with size-exclusion chromatography over Sephadex G-10, and… CONTINUE READING