Fractionated tritium-labelled heparin studied in vitro and in vivo.

@article{Shanberge1978FractionatedTH,
  title={Fractionated tritium-labelled heparin studied in vitro and in vivo.},
  author={J. Shanberge and M. Gruhl and T. Kitani and S. Ambegaonkar and J. Kambayashi and M. Nakagawa and D. Lenter},
  journal={Thrombosis research},
  year={1978},
  volume={13 5},
  pages={
          767-83
        }
}
Abstract Tritium-labelled commercially prepared porcine mucosal heparin was fractionated by chromatography on Sephadex G-200. The fractions obtained were studied for sulfate content, metachromasia, and ability to complex with antithrombin to produce anticoagulant activity. When added to platelet poor plasma and rechromatographed most of the higher molecular weight fractions were found between the globulin and albumin protein peaks combined with antithrombin. The fractions were grouped into… Expand
17 Citations
Disposition of fractionated 3H-heparin in rats.
TLDR
It was suggested by gel filtration chromatography and affinity chromatography that the main material of the radioactivity excreted in urine in 5 h has the same properties as that of the administered heparin fraction. Expand
Disposition of tritium-labelled heparin in rats.
TLDR
Whole-body autoradiograms showed that the reticulo-endotherial system (R.E.S.) may play an important role in removal of the radioactivity of heparin from the circulating blood. Expand
Dose-dependent disposition of fractionated 3H-heparin in rats.
TLDR
It was suggested that tissue (liver) distribution of radioactivity might be a predominant factor for the dose-dependent disappearance of the radioactivity from plasma at an early stage following the administration of fractionated 3H-heparin. Expand
Gel chromatography of heparin.
TLDR
A column chromatographic technique for the fractionation of heparin is described using various diameters of bead form cross-linked dextran gels and an automated apparatus and it was observed that Sephadex G-50 resulted in the separation of three well formed peaks and provided superior resolution compared to all other gels. Expand
THE INTERACTION OF PROTEIN‐BOUND HEPARIN AND ANTITHROMBIN III
TLDR
Observations presented may help explain heparin "rebound" in patients believed adequately neutralized with protamine, and help explain the role of serine proteases in the response to protamine. Expand
Metachromatic activity of heparin and heparin fragments.
TLDR
Heparin of an average molecular weight of 13,000 was fractionated on the basis of size into five fractions of different weight-average molecular weight ranging from 8500 to 20,000, and metachromatic activity was found in a fragment as small as a tetrasaccharide. Expand
Indium-111 DTPA-heparin: radiolabeling, pharmacokinetics, and biodistribution following intravenous administration in rat and rabbit.
Heparin was coupled to DTPA using the bicyclic anhydride and labeled with Indium-111. This resulted in a radiochemically pure preparation (greater than 95% activity in one peak) as determined by highExpand
Clinical Pharmacokinetics of Heparin
  • J. Estes
  • Chemistry, Medicine
  • Clinical pharmacokinetics
  • 1980
TLDR
It would appear that optimum therapy with heparin can be achieved only when the individual patient’s response to, and rate of elimination ofHeparin are taken into account concurrently. Expand
Synthetic analogues of the antithrombin III-binding pentasaccharide sequence of heparin. Prediction of in vivo residence times.
TLDR
It is hypothesized that the elimination half-life of pentasaccharides is markedly extended by ATIII binding, of which the extent is governed by the Kd of the complex, and the following observations support this hypothesis. Expand
Effect of protamine on heparin-antithrombin III complexes. In vitro studies.
Abstract In vitro, when protamine sulfate (salmine) is added to heparinized human plasma, the activated heparin-antithrombin III complex which had been formed is broken, freeing the antithrombin andExpand
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