Fraction of ribosomes synthesizing protein as a function of specific growth rate.


The fraction of ribosomes engaged in protein synthesis in Escherichia coli growing at specific growth rates ranging from 0.3 to 1.2 h(-1) was estimated from measurements of nascent protein/ribosome and of polyribosomes. Both measurements showed that the fraction of ribosomes synthesizing protein increased with specific growth rate, from 30 to 70% over the… (More)