Fractal assembly of micrometre-scale DNA origami arrays with arbitrary patterns

  title={Fractal assembly of micrometre-scale DNA origami arrays with arbitrary patterns},
  author={Grigory Tikhomirov and Philip Petersen and Lulu Qian},
Self-assembled DNA nanostructures enable nanometre-precise patterning that can be used to create programmable molecular machines and arrays of functional materials. [] Key Method We illustrate this method, which we term ‘fractal assembly’, by producing DNA origami arrays with sizes of up to 0.5 square micrometres and with up to 8,704 pixels, allowing us to render images such as the Mona Lisa and a rooster. We find that self-assembly of the tiles into arrays is unaffected by changes in surface patterns on the…

Constructing Large 2D Lattices Out of DNA-Tiles

This review first summarizes currently available DNA tile toolboxes and the basic principles of lattice formation and hierarchical self-assembly using DNA tiles, and focuses on the recent approaches that have shown great potential for the controlled immobilization and positioning of DNA nanostructures on different surfaces.

Triangular DNA Origami Tilings.

The programmability of the edge design and the flexibility of the structure make the triangular DNA origami tile an ideal building block for complex self-assembly and reconfiguration in artificial molecular machines and fabricated nanodevices.

Multiform DNA origami arrays using minimal logic control.

This study investigated the feasibility of using the minimum pairs of DNA connection strands to implement algorithm-based self-assembly with finite DNA origamis and found that the DNA origami linking complexity was markedly reduced.

Multi-micron crisscross structures from combinatorially assembled DNA-origami slats

Crisscross polymerization of DNA-origami slats is introduced for strictly seed-dependent growth of custom multi-micron shapes with user-defined nanoscale surface patterning and provides a generalizable route for prototyping and scalable production of devices integrating thousands of unique components that each are sophisticated and molecularly precise.

Complex wireframe DNA nanostructures from simple building blocks

A design paradigm that circumvents the sophisticated routing and size limitations intrinsic to the scaffold strand in DNA origami is demonstrated, which produces a myriad of wireframe structures, including 2D arrays, tubes, polyhedra, and multi-layer 3D arrays.

Programming 2D Supramolecular Assemblies with Wireframe DNA Origami.

Wireframe DNA origami offers the ability to program nearly arbitrary 2D and 3D nanoscale geometries, with six-helix bundle (6HB) edge designs providing both geometric versatility and fidelity with

Reciprocal Control of Hierarchical DNA Origami-Nanoparticle Assemblies.

It is shown that the angular distributions of DNA origami hinge mechanisms are tunable as a function of nanoparticle size and distance from the hinge vertex, which provides guiding principles toward the design of dynamic, DNA-origami hierarchical materials capable of storing and releasing mechanical energy.

Hierarchical Assembly of Super-DNA Origami Based on a Flexible and Covalent-Bound Branched DNA Structure.

A general strategy to efficiently organize multiple DNA origami tiles to form super-DNA origami using a flexible and covalent-bound branched DNA structure that presents a new avenue for the construction of sophisticated DNA architectures with larger molecular weights.

Meta-DNA structures

It is demonstrated that a six-helix bundle DNA origami nanostructure in the submicrometre scale ( meta-DNA) could be used as a magnified analogue of single-stranded DNA, and that two meta-DNAs that contain complementary ‘meta-base pairs’ can form double helices with programmed handedness and helical pitches.

Autonomously designed free-form 2D DNA origami

This work presents a fully autonomous procedure to design all DNA staple sequences needed to fold any free-form 2D scaffolded DNA origami wireframe object and enables the full autonomy of scaffold routing and staple sequence design with arbitrary network edge lengths and vertex angles.



Finite-size, fully addressable DNA tile lattices formed by hierarchical assembly procedures.

Higher production yields of defect-free assemblies were achieved by procedures that minimize assembly depth (and maximize diversity of address labels) and some observations on scaling of these strategies to larger arrays are also presented.

Rapid prototyping of 3D DNA-origami shapes with caDNAno

DNA nanotechnology exploits the programmable specificity afforded by base-pairing to produce self-assembling macromolecular objects of custom shape. For building megadalton-scale DNA nanostructures,

Organizing DNA origami tiles into larger structures using preformed scaffold frames.

This method uses a collection of bridge strands to prefold a single-stranded DNA scaffold into a loose framework, and demonstrates the ability to organize DNA origami nanostructures into larger spatially addressable architectures.

Folding DNA to create nanoscale shapes and patterns

This work describes a simple method for folding long, single-stranded DNA molecules into arbitrary two-dimensional shapes, which can be programmed to bear complex patterns such as words and images on their surfaces.

Complex shapes self-assembled from single-stranded DNA tiles

This work implements the strategy with a master strand collection that corresponds to a 310-pixel canvas, and uses appropriate strand subsets to construct 107 distinct and complex two-dimensional shapes, thereby establishing SST assembly as a simple, modular and robust framework for constructing nanostructures with prescribed shapes from short synthetic DNA strands.

Toward larger DNA origami.

Very efficient assembly of a 51-kilobasepair origami from the λ/M13 hybrid scaffold folded by chip-derived staple strands is experimentally demonstrated and two-dimensional, asymmetric origami sheets with controlled global curvature such that they land on a substrate in predictable orientations that have been verified by atomic force microscopy.

Self-assembly of carbon nanotubes into two-dimensional geometries using DNA origami templates.

A general method for arranging single-walled carbon nanotubes in two dimensions using DNA origami-a technique in which a long single strand of DNA is folded into a predetermined shape to allow the rapid prototyping of complex nanotube-based structures.

Programmable disorder in random DNA tilings.

A framework for programming random DNA tilings and how to control the properties of global patterns through simple, local rules is demonstrated and new opportunities for fabricating more complex molecular devices that are organized by DNA nanostructures are created.

A Proximity-Based Programmable DNA Nanoscale Assembly Line

It is demonstrated that a nanoscale assembly line can be realized by the judicious combination of three known DNA-based modules: a DNA origami tile that provides a framework and track for the assembly process, cassettes containing three independently controlled two-state DNA machines that serve as programmable cargo-donating devices and a DNA walker that can move on the track from device to device and collect cargo.

Hierarchical assembly of metal nanoparticles, quantum dots and organic dyes using DNA origami scaffolds.

R rigid DNA origami scaffolds can be used to assemble metal nanoparticles, quantum dots and organic dyes into hierarchical nanoclusters that have a planet-satellite-type structure and can be positioned along the radial DNA spacers of the nanostructures.