Fourier transform infrared microspectroscopy identifies symmetric PO(2)(-) modifications as a marker of the putative stem cell region of human intestinal crypts.

@article{Walsh2008FourierTI,
  title={Fourier transform infrared microspectroscopy identifies symmetric PO(2)(-) modifications as a marker of the putative stem cell region of human intestinal crypts.},
  author={Michael J. Walsh and Tariq G Fellous and Azzedine Hammiche and Wey-Ran Lin and Nigel J Fullwood and Olaug Grude and Fariba Bahrami and James M. Nicholson and Marine Cotte and Jean Susini and Hubert M. Pollock and Mairi Brittan and Pierre Leonard Martin-Hirsch and Malcolm R Alison and Francis Luke Martin},
  journal={Stem cells},
  year={2008},
  volume={26 1},
  pages={108-18}
}
Complex biomolecules absorb in the mid-infrared (lambda = 2-20 microm), giving vibrational spectra associated with structure and function. We used Fourier transform infrared (FTIR) microspectroscopy to "fingerprint" locations along the length of human small and large intestinal crypts. Paraffin-embedded slices of normal human gut were sectioned (10 microm thick) and mounted to facilitate infrared (IR) spectral analyses. IR spectra were collected using globar (15 microm x 15 microm aperture… CONTINUE READING

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