Fourier analysis of differential light scattering for the quantitation of FSH response associated with structural changes in immortalized granulosa cells

@article{Schiffer1996FourierAO,
  title={Fourier analysis of differential light scattering for the quantitation of FSH response associated with structural changes in immortalized granulosa cells},
  author={Ze'ev Schiffer and Iris Keren-Tal and Motti Deutsch and Ada Dantes and Abraham Amsterdam},
  journal={Molecular and Cellular Endocrinology},
  year={1996},
  volume={118},
  pages={145-153}
}
We have established granulosa cell lines which express constitutively the rat FSH receptors by cotransfection of primary granulosa cells obtained from preovulatory follicles with SV40 DNA, Ha-ras oncogene and a plasmid expressing FSH receptors. These cells respond specifically to ovine and human FSH by cell rounding, intracellular cAMP accumulation, and progesterone secretion in a dose-dependent manner. A new method for the demonstration and quantitation of changes in cell shape-Small Angle… CONTINUE READING