Four disulfide-bridged scorpion beta neurotoxin CssII : heterologous expression and proper folding in vitro

@inproceedings{Georgina2009FourDS,
  title={Four disulfide-bridged scorpion beta neurotoxin CssII : heterologous expression and proper folding in vitro},
  author={Estrada Georgina and Schiavon Emanuele and Ortiz Ernesto and Wanke Enzo and Possani Lourival and Corzo Gerardo},
  year={2009}
}
The gene of the four disulfide-bridged toxin II was cloned into the expression vector pQE30 Centruroides suffusus suffusus containing a 6His-tag and an FXa proteolytic cleavage region. This recombinant vector was transfected into BL21 cells and E. coli expressed under induction with isopropyl thiogalactoside (IPTG). The level of expression was 24.6 mg/L of culture medium, and the His tagged recombinant toxin (HisrCssII) was found exclusively in inclusion bodies. After solubilization the… CONTINUE READING