Four-dimensional imaging of living chondrocytes in cartilage using confocal microscopy: a pragmatic approach.

@article{Errington1997FourdimensionalIO,
  title={Four-dimensional imaging of living chondrocytes in cartilage using confocal microscopy: a pragmatic approach.},
  author={Rachel J. Errington and Mark D. Fricker and J L Wood and Andrew C Hall and Nathan S. White},
  journal={The American journal of physiology},
  year={1997},
  volume={272 3 Pt 1},
  pages={C1040-51}
}
Regulation of cell volume is a fundamental cellular homeostatic mechanism in the face of osmotic stress. In normal articular cartilage, chondrocytes are exposed to a changing osmotic environment. We present a comprehensive protocol for studying the volume regulatory behavior of chondrocytes within intact cartilage tissue using confocal laser-scanning microscopy. Our data acquisition regime optimizes both signal-to-noise and cell viability during time-lapsed three-dimensional (3-D) (x, y, z, t… CONTINUE READING
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