Four-color flow cytometric detection of retrovirally expressed red, yellow, green, and cyan fluorescent proteins.

@article{Hawley2001FourcolorFC,
  title={Four-color flow cytometric detection of retrovirally expressed red, yellow, green, and cyan fluorescent proteins.},
  author={Teresa S. Hawley Robert G. Hawley and William G. Telford and Ahmad Ramezani and Robert G. Hawley},
  journal={BioTechniques},
  year={2001},
  volume={30 5},
  pages={
          1028-34
        }
}
Flow cytometric procedures are described to detect a "humanized" version of a new red fluorescent protein (DsRed) from the coral Discosoma sp. in conjunction with various combinations of three Aequorea victoria green fluorescent protein (GFP) variants--EYFP, EGFP, and ECFP. In spite of overlapping emission spectra, the combination of DsRed with EYFP, EGFP, and ECFP generated fluorescence signals that could be electronically compensated in real time using dual-laser excitation at 458 and 568 nm… CONTINUE READING

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