Fot1, a new family of fungal transposable elements

  title={Fot1, a new family of fungal transposable elements},
  author={M. J. Daboussi and Thierry Langin and Yves Brygoo},
  journal={Molecular and General Genetics MGG},
SummaryWe report here the discovery of a family of transposable elements, which we refer to as Fotl elements, in the fungal plant pathogen Fusarium oxysporum. The first element was identified as an insertion in the gene encoding nitrate reductase. It is 1928 by long, has 44 by inverted terminal repeats, contains a large open reading frame and is flanked by a 2 by (TA) target site duplication. This element shares significant structural similarities with a class of transposons that includes Tc1… 

Pot2, an inverted repeat transposon from the rice blast fungus Magnaporthe grisea

The cloning and characterisation of Pot2, a putative transposable element from Magnaporthe grisea, showed extensive identity to that of Fott, a transposability element from another phytopathogenic fungus, Fusarium oxysporum.

Transposition of a fungal MITE through the action of a Tc1 -like transposase

It is shown that the mimp1/impala double component system is fully functional in the heterologous species F. graminearum allowing the development of a highly efficient tool for gene-tagging in filamentous fungi.

The transposable element impala, a fungal member of the Tc1-mariner superfamily

The overall comparison shows that impala occupies an intermediate position between the mariner and Tcl-like elements, suggesting that all these elements belong to the same superfamily.

Transposable elements in the fungal plant pathogenFusarium oxysporum

Comparisons of the distribution of transposable elements in Fusarium populations have improved the understanding of population structure and epidemiology and provided support for horizontal genetic transfer.

The Tc1/mariner Family of Transposable Elements

There are two reasons to think that Tc1/mariner-type transposons may be good vectors for transgenesis: they have spread by horizontal transfer between species and are thus probably not host restricted, and they require only the transposase protein for complete transposition.

The biology and potential for genetic research of transposable elements in filamentous fungi

The application of transposable elements to gene isolation and population analysis is an important tool for molecular biology and studies of fungal evolution.

The isolation of Ant1, a transposable element from Aspergillus niger

Sequence homology and structural features indicate that this transposable element, which has been named Ant1 (A. niger transposon 1), is related to the Tc1/mariner group of DNA transposons.

Transposable elements in filamentous fungi.

All eukaryotic TEs described are found including an extraordinary prevalence of active members of the pogo family, leading to significant advances in perception of the mechanisms underlying genetic changes in these organisms.

Occan, a novel transposon in the Fot1 family, is ubiquitously found in several Magnaporthe grisea isolates

Phylogenetic analysis with other known members of the Fot1 family revealed that Occan was dissimilar to any other known elements and it is proposed that occan be separated to a new subfamily.



Isolation of a transposable element from Neurospora crassa.

  • J. KinseyJ. Helber
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1989
A Neurospora crassa strain from Adiopodoumé, Ivory Coast, contains multiple copies of a transposable element, Tad. The element was detected as a 7-kilobase insertion in two independently isolated

The transposable element Uhu from Hawaiian Drosophila--member of the widely dispersed class of Tc1-like transposons.

The complete nucleotide sequence of the transposable element Uhu is reported from the vicinity of the alcohol dehydrogenase (Adh) gene of Drosophila heteroneura (an endemic Hawaiian Drosophile) and it is demonstrated that Uhu belongs to a class of transposables elements which includes Tc1 from Caenorhabditis elegans, Barney fromCaenor Habditis briggsae, and HB1 from Drosophon melanogaster.

Sequence of the C. elegans transposable element Tc1.

The complete nucleotide sequence was determined for Tc1, a transposable element in the nematode Caenorhabditis elegans, which contains two long open reading frames on the same DNA strand but in different translational reading frames, suggesting that a single transcript is made.

Molecular structure of a somatically unstable transposable element in Drosophila.

The unstable white-peach (wpch) allele exhibits a spectrum of germ-line and somatic mutability more similar to insertion mutations in maize and in the nematode Caenorhabditis elegans than has been reported for insertion mutation mutations in Drosophila.

Sequence identity between an inverted repeat family of transposable elements in Drosophila and Caenorhabditis

It is demonstrated that Tc1 shares sequence identity in its open reading frame and terminal repeats with a new transposable element Barney (also known as TCb1-Transposon Caenorhabditis briggsae 1).

Tnt1, a mobile retroviral-like transposable element of tobacco isolated by plant cell genetics

Tntl has been isolated after its transposition into the nitrate reductase structural gene of tobacco, and transposition events have been detected through in vitro selection of spontaneous NR-deficient (NR–) mutant lines in cell cultures derived from tobacco mesophyll protoplasts.

Evidence for a transposon in caenorhabditis elegans

Insertion and excision of the transposable element mariner in Drosophila.

Mariner excision is imprecise in both germline and somatic cells of Drosophila mauritiana, and the most frequent excision events are the same in the two tissues.